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In predicting allosteric sites, STINGAllo highlights the pivotal role of the internal protein nanoenvironment in defining the allosteric potential of residues. Traditional methods often focus on identifying pockets, which can miss crucial spatial and physicochemical contexts. StingAllo, however, employs a per-residue approach, in analyzing the nanoenvironment surrounding each residue, ensuring a more accurate prediction of allosteric forming residues (AFRs), surpassing the capabilities of pocket-focused techniques.
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